Quantitative image analyses of nuclear dynamics in migrating neurons

In this paper, we present a novel method to investigate the mechanism driving nuclear translocation in migrating neurons via digital image analyses of the nucleus. We obtained and quantitatively analyzed time-lapse images of the fluorescently labeled nucleus with a high-speed confocal microscope.

We focused on spatiotemporal dynamics of the boundary curvature of the nucleus during nuclear translocation. Our data suggest major contribution of a pulling force to nuclear translocation during neuronal migration.

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