In this paper, we present a novel method to investigate the mechanism driving nuclear translocation in migrating neurons via digital image analyses of the nucleus. We obtained and quantitatively analyzed time-lapse images of the fluorescently labeled nucleus with a high-speed confocal microscope.
We focused on spatiotemporal dynamics of the boundary curvature of the nucleus during nuclear translocation. Our data suggest major contribution of a pulling force to nuclear translocation during neuronal migration.